The resulting strains induced a resistant response in a cultivar-specific manner, suggesting that similar resistance mechanisms may function in Arabidopsis and soybean. syringae pv glycinea to test whether this locus could determine avirulence on soybean. The avrRpt2 locus from Pst was also moved into virulent strains of the soybean pathogen P. As a first step toward genetically mapping the postulated resistance locus, an ecotype susceptible to infection by DC 3000 carrying avrRpt2 was identified. We found that Arabidopsis ecotype Col-0 was susceptible to Pst strain DC 3000 but resistant to the same strain carrying avrRpt2, suggesting that a single locus in Col-0 determines resistance. This allowed us to construct pathogens that differed only by the presence or absence of a single putative avirulence gene. Cosmids that determine avirulence were isolated from Pst genomic libraries, and the Pst avirulence locus avrRpt2 was defined.

Therefore, we tested whether avirulence genes in Pst controlled induction of resistance in Arabidopsis. In many plant-pathogen interactions, disease resistance is controlled by the simultaneous presence of single plant resistance genes and single pathogen avirulence genes. Pst strains were found to be virulent or avirulent on specific Arabidopsis ecotypes, and single ecotypes were resistant to some Pst strains and susceptible to others. To develop a model system for molecular genetic analysis of plant-pathogen interactions, we studied the interaction between Arabidopsis thaliana and the bacterial pathogen Pseudomonas syringae pv tomato ( Pst). Whalen, M C Innes, R W Bent, A F Staskawicz, B J Identification of Pseudomonas syringae pathogens of Arabidopsis and a bacterial locus determining avirulence on both Arabidopsis and soybean.

Adam audio s5h frequency response chart